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42-491 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-491 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-491 (0.1ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ATP1B1 Antibody

CATALOG NUMBER: 42-491

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-KTEISFRPNDPKSYE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Rat Retina lysates and 50kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1kDa according to Human NP_001668.1, 35.2kDa according to Mouse NP_033851.1 and Rat NP_037245.2).The observed molecular weight corresponds to the glycosylated form (PMID: 20130120). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
Positive Control 1:
Rat Retina Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATP1B1
Additional Names:
ATP1B1, ATPase, Na+/K+ transporting, beta 1 polypeptide, ATP1B, MGC1798, Beta 1-subunit of Na(+),K(+)-ATPase, Na+/K+ -ATPase beta 1 subunit, Na, K-ATPase beta-1 polypeptide, Na,K-ATPase beta 1 subunit, OTTHUMP00000032537, OTTHUMP00000032538, adenosinetriphosphatase, sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase beta-1 chain
Protein Accession Number:
NP_001668.1,NP_001001787.1
Protein Gi Number:
4502277
Ncbi Gene Id Number:
481

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-491

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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