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42-526 (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-526 (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-526 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Adipose lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-526 (0.01ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-526 (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ACO2 Antibody

CATALOG NUMBER: 42-526

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QDTYQHPPKDSSGQH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACO2
Additional Names:
ACO2, aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908,aconitate hydratase, citrate hydro-lyase
Protein Accession Number:
NP_001089.1
Protein Gi Number:
4501867
Ncbi Gene Id Number:
50

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-526

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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