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42-720 (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-720 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-720 (0.1ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Ascl3 Antibody

CATALOG NUMBER: 42-720

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-HLPEDYLEKRLSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Ascl3
Additional Names:
Ascl3, achaete-scute complex homolog 3 (Drosophila), MGC129412, MGC129413, Sgn1, bHLHa42, ASH-3, ICRFP703B1614Q5.4, ICRFP703N2430Q5.4, achaete-scute complex homolog-like 3, achaete-scute homolog 3, bHLH transcriptional regulator Sgn-1, mASH-3, mASH3
Protein Accession Number:
NP_064435.1
Protein Gi Number:
9910134
Ncbi Gene Id Number:
56676

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-720

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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