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42-774 (1.5ug/ml) as the reporter antibody.
42-774 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-774 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-774 (1.5ug/ml) as the reporter antibody.

CANX Antibody

CATALOG NUMBER: 42-774

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificity:
Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 4:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CANX
Additional Names:
CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88
Protein Accession Number:
NP_001737.1
Protein Gi Number:
10716563
Ncbi Gene Id Number:
821

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-774

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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