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42-836 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-836 (3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-836 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SGCD Antibody

CATALOG NUMBER: 42-836

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RLPHGSYTPTGTR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SGCD
Additional Names:
SGCD, sarcoglycan, delta (35kDa dystrophin-associated glycoprotein), 35DAG, CMD1L, DAGD, MGC22567, SG-delta, SGCDP, SGD, 35 kDa dystrophin-associated glycoprotein, 35kD dystrophin-associated glycoprotein, delta-SG, delta-sarcoglycan, dystrophin associated glycoprotein, delta sarcoglycan, placental delta sarcoglycan
Protein Accession Number:
NP_000328.2,NP_758447.1,NP_001121681.1
Protein Gi Number:
21314619
Ncbi Gene Id Number:
6444

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-836

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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