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42-853 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-853 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-853 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

APOBEC3D Antibody

CATALOG NUMBER: 42-853

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PKRQSNHRQEVYFR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in lysates of cell line A549 (calculated MW of 46.6kDa according to NP_689639.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APOBEC3D
Additional Names:
APOBEC3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D, APOBEC3DE, APOBEC3E, ARP6, DNA dC->dU-editing enzyme APOBEC-3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3E pseudogene, probable DNA dC->dU-editing enzyme APOBEC-3D
Protein Accession Number:
NP_689639.2
Protein Gi Number:
187608816
Ncbi Gene Id Number:
140564

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-853

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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