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42-879 (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-879 (5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-879 (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TPO Antibody

CATALOG NUMBER: 42-879

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TRHVIQVSNEVVTDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms a (NP_000538.3), b (NP_783650.1), d (NP_78652.1), and e (NP_783653.1).
Positive Control 1:
Cat. No. 1350 - Human Thyroid Tissue Lysate
Predicted Molecular Weight:
Approx 110 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TPO
Additional Names:
TPO, thyroid peroxidase, MSA, TDH2A, TPX, OTTHUMP00000115535, OTTHUMP00000115536, thyroid microsomal antigen, thyroperoxidase
Protein Accession Number:
NP_000538.3,NP_783650.1,NP_783652.1,NP_783653.1
Protein Gi Number:
28558982
Ncbi Gene Id Number:
7173

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-879

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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