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42-898 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-898 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
42-898 (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

App Antibody

CATALOG NUMBER: 42-898

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: DAEFGHDSGFEVRHQK-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Mouse Brain lysates (calculated MW of 86.7kDa according to NP_001185752.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1; NP_031497.2; NP_001185753.1; NP_001185754.1; NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Approx 100 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
App
Additional Names:
App, amyloid beta (A4) precursor protein, Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp, alzheimer disease amyloid A4 protein homolog, amyloid beta A4 protein, amyloidogenic glycoprotein, appican, protease nexin II
Protein Accession Number:
NP_001185752.1,NP_031497.2,NP_001185753.1,NP_001185754.1,NP_001185755.1
Protein Gi Number:
311893401
Ncbi Gene Id Number:
11820(mouse);54226(rat);

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-898

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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