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43-123 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-123 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-123 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SH2D3A / NSP1 Antibody

CATALOG NUMBER: 43-123

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-APRAERFEKFQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SH2D3A / NSP1
Additional Names:
SH2D3A, NSP1, SH2 domain containing 3A, SH2 domain-containing 3A, novel SH2-containing protein 1
Protein Accession Number:
NP_005481.2
Protein Gi Number:
208609985
Ncbi Gene Id Number:
10045
Background Reference 1:
Near RI, Zhang Y, Makkinje A, Vanden Borre P, Lerner A.AND-34/BCAR3 differs from other NSP homologs in induction of anti-estrogen resistance, cyclin D1 promoter activation and altered breast cancer cell morphology.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-123

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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