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43-185 (1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-185 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-185 (1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ZBTB24 Antibody

CATALOG NUMBER: 43-185

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PVEKDENYDPKTED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 80kDa band observed in lysates of cell line Daudi (calculated MW of 78.3kDa according to NP_055612.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_055612.2; NP_001157785.1).
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZBTB24
Additional Names:
ZBTB24, zinc finger and BTB domain containing 24, BIF1, ICF2, PATZ2, ZNF450, OTTHUMP00000016968, POZ (BTB) and AT hook containing zinc finger 2, zinc finger and BTB domain-containing protein 24, zinc finger protein 450
Protein Accession Number:
NP_055612.2,NP_001157785.1
Protein Gi Number:
256600206
Ncbi Gene Id Number:
9841
Background Reference 1:
de Greef JC, Wang J, Balog J, den Dunnen JT, Frants RR, Straasheijm KR, Aytekin C, van der Burg M, Duprez L, Ferster A, Gennery AR, Gimelli G, Reisli I, Schuetz C, Schulz A, Smeets DF, Sznajer Y, Wijmenga C, van Eggermond MC, van Ostaijen-Ten Dam MM, LankMutations in ZBTB24 are associated with immunodeficiency, centromeric instability, and facial anomalies syndrome type 2

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-185

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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