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43-226 (0.3ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-226 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-226 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-226 (0.3ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Glycine receptor, alpha 1 Antibody

CATALOG NUMBER: 43-226

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CLQAKDGISVKGAN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Kidney. Recommended concentration: 5ug/ml.
Specificity:
The immunizing peptide represents part of the cytoplasmic domain. The antibody is not expected to cross-react with other glycine receptor alpha proteins.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Glycine receptor, alpha 1
Additional Names:
Glra1, glycine receptor, alpha 1, GLYRA1, glycine receptor 48 kDa subunit, glycine receptor strychnine-binding subunit, glycine receptor subunit alpha-1, glycine receptor, alpha 1 subunit
Protein Accession Number:
NP_037265.1
Protein Gi Number:
119372310
Ncbi Gene Id Number:
2741
Background Reference 1:
Wang Q, Lynch JW.Activation and desensitization induce distinct conformational changes at the extracellular-transmembrane domain interface of the glycine receptor.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-226

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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