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43-378 (2ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-378 (2ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

periostin Antibody

CATALOG NUMBER: 43-378

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RKLQANKKVQGSRR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 100kDa band observed in lysates of cell line MCF7 (calculated MW of 93.3kDa according to NP_006466.2). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_006466.2; NP_001129406.1; NP_001129407.1; NP_001129408.1).
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
periostin
Additional Names:
POSTN, periostin, osteoblast specific factor, MGC119510, MGC119511, OSF-2, OSF2, PDLPOSTN, PN, RP11-412K4.1, periostin, OTTHUMP00000018268, OTTHUMP00000018269, OTTHUMP00000018270, OTTHUMP00000018271, osteoblast specific factor 2 (fasciclin I-like), osteoblast-specific factor 2, periodontal ligament-specific periostin, periostin, periostin isoform thy2, periostin isoform thy4, periostin isoform thy6, periostin isoform thy8
Protein Accession Number:
NP_006466.2,NP_001129406.1,NP_001129407.1,NP_001129408.1
Protein Gi Number:
209862907
Ncbi Gene Id Number:
10631
Background Reference 1:
Sen K, Lindenmeyer MT, Gaspert A, Eichinger F, Neusser MA, Kretzler M, Segerer S, Cohen CD.Periostin is induced in glomerular injury and expressed de novo in interstitial renal fibrosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-378

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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