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43-430 (0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-430 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-430 (0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

BAG3 Antibody

CATALOG NUMBER: 43-430

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KNAGNAEDPHTE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Pancreas lysates (calculated MW of 61.6kDa according to NP_004272.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAG3
Additional Names:
BAG3, BCL2-associated athanogene 3 , BAG-3, BIS, CAIR-1, MGC104307, BAG-family molecular chaperone regulator-3, BCL2-binding athanogene 3, Bcl-2-binding protein, docking protein CAIR-1
Protein Accession Number:
NP_004272.2
Protein Gi Number:
14043024
Ncbi Gene Id Number:
9531
Background Reference 1:
Homma S, Iwasaki M, Shelton GD, Engvall E, Reed JC, Takayama S.BAG3 deficiency results in fulminant myopathy and early lethality.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-430

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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