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46-528 staining (0.003ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
46-528 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
46-528 staining (0.003ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

Triosephosphate isomerase Antibody

CATALOG NUMBER: 46-528

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LKPEFVDIINAKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in Human Liver (calculated MW of 26.7kDa according to NP_000356.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubated for 1 hour.Immunohistochemistry:In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise reported isoforms 1 and 2 (NP_000356.1; NP_001152759.1).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TPI1
Additional Names:
TPI1, triosephosphate isomerase 1, TPI, MGC88108
Protein Accession Number:
NP_000356.1,NP_001152759.1
Protein Gi Number:
4507645
Ncbi Gene Id Number:
7167
Background Reference 1:
Jiang PZ, Gan M, Huang H, Shen XM, Wang S, Yao KT. Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells. Acta Biochim Biophys Sin (Shanghai). 2005 Feb;37(2):97-106.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-528

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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