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43-459 (0.3ug/ml) staining of Albumin-depleted Human Plasma lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-459 (0.3ug/ml) staining of Albumin-depleted Human Plasma lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

alpha-1-antitrypsin Antibody

CATALOG NUMBER: 43-459

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QKTDTSHHDQDHP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Albumin-depleted Human Plasma lysates (calculated MW of 46.7kDa according to NP_000286.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Specificity:
The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_001121174.1, NP_001121179.1, NP_001121172.1, NP_001121177.1, NP_001121178.1, NP_001121176.1, NP_001121175.1, NP_000286.3, NP_001121173.1, NP_001002236.1, NP_001002235.1.
Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
alpha-1-antitrypsin
Additional Names:
SERPINA1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1, A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, alpha-1 protease inhibitor, alpha-1-antiproteinase, alpha-1-antitrypsin, alpha-1-antitrypsin null, protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin, serine (or cysteine) proteinase inhibitor, clade A, member 1, serpin A1
Protein Accession Number:
NP_000286.3
Protein Gi Number:
50363217
Ncbi Gene Id Number:
5265
Background Reference 1:
Tsutsui Y, Dela Cruz R, Wintrode PL.Folding mechanism of the metastable serpin α1-antitrypsin.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-459

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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