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43-645 (0.3ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-645 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-645 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-645 (0.3ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ASIC1 Antibody

CATALOG NUMBER: 43-645

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NILPHHPARGT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Mouse fetal Brain lysates (calculated MW of 64.8kDa according to NP_064423.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Pancreas. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_064423.2; NP_001086.2; NP_001243759.1).
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ASIC1
Additional Names:
ASIC1, acid-sensing (proton-gated) ion channel 1, ACCN2, ASIC, BNaC2, Cation channel, amiloride-sensitive, neuronal, 2, acid-sensing ion channel 1, acid-sensing ion channel 1a protein, amiloride-sensitive cation channel 2, neuronal, brain sodium channel 2
Protein Accession Number:
NP_064423.2,NP_001086.2,NP_001243759.1
Protein Gi Number:
21536351
Ncbi Gene Id Number:
41
Background Reference 1:
Hoagland EN, Sherwood TW, Lee KG, Walker CJ, Askwith CC.Identification of a calcium permeable human acid-sensing ion channel 1 transcript variant.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-645

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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