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43-671 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-671 (0.1ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-671 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

cardiac troponin T Antibody

CATALOG NUMBER: 43-671

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TERKSGKRQTERE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human, Mouse and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID: 20083571). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1).
Predicted Molecular Weight:
Approx 40kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
cardiac troponin T
Additional Names:
TNNT2, troponin T type 2 (cardiac), CMH2, CMPD2, LVNC6, MGC3889, RCM3, TnTC, cTnT, OTTHUMP00000033864, OTTHUMP00000033865, OTTHUMP00000033866, OTTHUMP00000033867, OTTHUMP00000033870, OTTHUMP00000218095, cardiac muscle troponin T, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, troponin T, cardiac muscle, troponin T2, cardiac
Protein Accession Number:
NP_000355.2,NP_001001430.1,NP_001001431.1,NP_001001432.1
Protein Gi Number:
21264536
Ncbi Gene Id Number:
7139
Background Reference 1:
Zhang J, Zhang H, Ayaz-Guner S, Chen YC, Dong X, Xu Q, Ge YPhosphorylation, but not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac troponin T.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-671

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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