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45-005 (1ug/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing AKT3 and probed with 45-005 (mock transfection in second lane).
45-005 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-005 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-005 (1ug/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AKT3 Antibody

CATALOG NUMBER: 45-005

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CSPTSQIDNIGEEEM
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:A 50-55kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0kDa according to NP_859029.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate and Heart. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKT3
Additional Names:
AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase, PKB-GAMMA, AKT3 kinase, OTTHUMP00000037911, OTTHUMP00000037912
Protein Accession Number:
NP_005456.1,NP_859029.1
Protein Gi Number:
4885549
Ncbi Gene Id Number:
10000
Background Reference 1:
Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res. 2001 Aug;7(8):2475-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-005

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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