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45-012 (1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-012 (1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

ATF2 Antibody

CATALOG NUMBER: 45-012

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-IVMAPSSQSQPSGS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562, which corresponds with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATF2
Additional Names:
ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Protein Accession Number:
NP_001871.2
Protein Gi Number:
22538422
Ncbi Gene Id Number:
1386
Background Reference 1:
Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol 2001 May 14;153(4):687-98

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-012

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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