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SMAD4 Antibody

CATALOG NUMBER: 45-148

Clonality:
Polyclonal
Tested Applications:
ELISA, IP
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Pig Cow
Immunogen:
The immunogen for this antibody is: C-HTMPIADPQPLD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave a band at approx 75kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literatureImmunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SMAD4
Additional Names:
MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, SMAD family member 4, OTTHUMP00000163548, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, mothers against decapentaplegic homolog 4
Protein Accession Number:
NP_005350.1
Protein Gi Number:
4885457
Ncbi Gene Id Number:
4089
Background Reference 1:
Kim SK, Fan Y, Papadimitrakopoulou V, Clayman G, Hittelman WN, Hong WK, Lotan R, Mao L. DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma. Cancer Res 1996 Jun 1;56(11):2519-21

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-148

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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