Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

45-170 (0.01ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-170 (1.5ug/ml) as the reporter antibody.
45-170 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-170 (0.01ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VHL Antibody

CATALOG NUMBER: 45-170

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-RSLVKPENYRRLD
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant VHL captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
Specificity:
This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VHL
Additional Names:
VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome
Protein Accession Number:
NP_000542.1,NP_937799.1
Protein Gi Number:
4507891
Ncbi Gene Id Number:
7428
Background Reference 1:
Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J. Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha. Mol Cell Biol. 2006 Mar;26(6):2019-28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-170

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369