Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

45-172 (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-172 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)
45-172 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue)
45-172 (1ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Wnt4 Antibody

CATALOG NUMBER: 45-172

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SNWLYLAKLSSVGS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Positive Control 2:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
WNT4
Additional Names:
WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein
Protein Accession Number:
NP_110388.2
Protein Gi Number:
17402922
Ncbi Gene Id Number:
54361
Background Reference 1:
Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell. Cancer Res. 1994 May 15;54(10):2615-21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-172

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369