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45-186 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-186 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-186 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-186 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle/ cytoplasmic staining. The nuc
45-186 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ABHD5 Antibody

CATALOG NUMBER: 45-186

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-FPERPDLADQDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2). Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.2-0.6ug/ml Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is not expected to cross-react with ABHD4.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ABHD5
Additional Names:
ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
Protein Accession Number:
NP_057090.2
Protein Gi Number:
31542303
Ncbi Gene Id Number:
51099
Background Reference 1:
Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome. Cell Metab. 2006 May;3(5):309-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-186

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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