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45-223 (0.02ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ARA9 and probed with 45-223 (mock transfection in first lane).
45-223 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-223 (0.02ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AIP Antibody

CATALOG NUMBER: 45-223

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EARIRQKDEEDKAR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.02-0.06ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIP
Additional Names:
AIP, ARA9, aryl hydrocarbon receptor interacting protein , FKBP16, FKBP37, XAP2 , HBV-X associated protein, aryl hydrocarbon receptor-interacting protein
Protein Accession Number:
NP_003968.1
Protein Gi Number:
4502009
Ncbi Gene Id Number:
9049
Background Reference 1:
Vierimaa O, Georgitsi M, Lehtonen R, Vahteristo P, Kokko A, Raitila A, Tuppurainen K, Ebeling TM, Salmela PI, Paschke R, Gundogdu S, De Menis E, Makinen MJ, Launonen V, Karhu A, Aaltonen LA. Pituitary adenoma predisposition caused by germline mutations in the AIP gene. Science. 2006 May 26;312(5777):1228-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-223

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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