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45-259 (4ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
45-259 (4ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

AP-2 gamma Antibody

CATALOG NUMBER: 45-259

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NKTLEKMEKHRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.
Positive Control 1:
Cat. No. 1376 - Human Skin Tissue Lysate
Positive Control 2:
Cat. No. 12-701 - Human Skin Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TFAP2C
Additional Names:
TFAP2C, ERF1, TFAP2G, hAP-2g, AP2-GAMMA, transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma), estrogen receptor factor 1, transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)
Protein Accession Number:
NP_003213.1
Protein Gi Number:
4507445
Ncbi Gene Id Number:
7022
Background Reference 1:
Hoei-Hansen CE, Rajpert-De Meyts E, Carlsen E, Almstrup K, Leffers H, Skakkebaek NE. Fast-track Article: A subfertile patient diagnosed with testicular carcinoma in situ by immunocytological staining for AP-2{gamma} in semen samples: Case report. Hum Reprod. 2005 Jan 13;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-259

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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