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45-261 (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-261 (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

APBB1 Antibody

CATALOG NUMBER: 45-261

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-GSLKPKRLGAHTP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APBB1
Additional Names:
APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR , adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein
Protein Accession Number:
NP_001155.1,NP_663722.1
Protein Gi Number:
4502131
Ncbi Gene Id Number:
322
Background Reference 1:
Hoe HS, Magill LA, Guenette S, Fu Z, Vicini S, Rebeck GW. FE65 Interaction with the apoE receptor ApoEr2. J Biol Chem. 2006 Apr 25;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-261

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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