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45-283 (2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-283 (2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

ARIH2 Antibody

CATALOG NUMBER: 45-283

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EQRRRTLLKDFHDT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3ug/ml. This antibody has also been successfully used in WB in PMID: 24486325.Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARIH2
Additional Names:
ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Protein Accession Number:
NP_006312.1
Protein Gi Number:
5453557
Ncbi Gene Id Number:
10425
Background Reference 1:
van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH. TRIADs: a new class of proteins with a novel cysteine-rich signature. Protein Sci. 1999 Jul;8(7):1557-61.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-283

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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