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45-288 (0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-288 (0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ARNO Antibody

CATALOG NUMBER: 45-288

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: EDGVYEPPDLTP-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates (calculated MW of 46.5kDa according to NP_004219.2 and NP_059431.1). Recommended concentration: 0.5-1.5ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_004219.2; NP_059431.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PSCD2
Additional Names:
ARNO, cytohesin 2, pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2), CTS18, CTS18.1, PSCD2L, SEC7L, Sec7p-L, Sec7p-like, ARF exchange factor, ARF nucleotide-binding site opener, pleckstrin homology, Sec7 and coiled-coil domains 2-like
Protein Accession Number:
NP_004219.2,NP_059431.1
Protein Gi Number:
47157332
Ncbi Gene Id Number:
9266
Background Reference 1:
Kolanus W. Guanine nucleotide exchange factors of the cytohesin family and their roles in signal transduction. Immunol Rev. 2007 Aug;218:102-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-288

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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