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46-378 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-378 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-378 (0.3ug/ml) staining of A549 (A) and U2OS (B) cell ysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-378 (0.1ug/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-378 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain is
46-378 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-378 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

SLC7A11 Antibody

CATALOG NUMBER: 46-378

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KGQTQNFKDAFSGRD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC7A11
Additional Names:
SLC7A11, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, CCBR1, xCT , cystine/glutamate transporter
Protein Accession Number:
NP_055146.1
Protein Gi Number:
7657683
Ncbi Gene Id Number:
23657
Background Reference 1:
Kaleeba JA, Berger EA. Kaposi's sarcoma-associated herpesvirus fusion-entry receptor: cystine transporter xCT. Science. 2006 Mar 31;311(5769):1921-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-378

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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