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45-311 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-311 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-311 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

B5 Receptor Antibody

CATALOG NUMBER: 45-311

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NAWKQNLNKVKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EIF3M
Additional Names:
EIF3M, B5 receptor, PCID1, PCI domain containing 1 (herpesvirus entry mediator), FLJ29030, GA17, hfl-B5, dendritic cell protein, fetal lung protein B5, eukaryotic translation initiation factor 3, subunit M, B5, PCID1
Protein Accession Number:
NP_006351.2
Protein Gi Number:
23397429
Ncbi Gene Id Number:
10480
Background Reference 1:
Perez-Romero P, Fuller AO. The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection. J Virol. 2005 Jun;79(12):7431-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-311

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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