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45-313 (0.03ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing BAALC and probed with 45-313 (mock transfection in first lane).
45-313 (0.03ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BAALC Antibody

CATALOG NUMBER: 45-313

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig
Immunogen:
The immunogen for this antibody is: C-KQNGLQTTEAKRDAK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 18kDa band observed in mouse brain lysates (calculated MW of 15.6kDa according to human NP_079088.1 and of 15.5kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1ug/ml.An approx, 48kDa band was consistently observed in both Human and Mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise isoform 1 (NP_079088.1) only.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAALC
Additional Names:
BAALC, brain and acute leukemia, cytoplasmic , HGNC:14333, FLJ12015
Protein Accession Number:
NP_079088.1
Protein Gi Number:
13376200
Ncbi Gene Id Number:
79870
Background Reference 1:
Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A. Baalc, a marker of mesoderm and muscle. Gene Expr Patterns. 2005 Apr;5(4):463-73.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-313

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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