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45-314 staining (1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-314 staining (1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

BAF53A Antibody

CATALOG NUMBER: 45-314

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: YEEGGKQCVERKCP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in HeLa lysates (predicted MW of 49kDa according to NP_057272.1, NP_004292.1). Recommended for use at 1-3ug/ml. Primary incubation was for 1 hour.
Specificity:
This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACTL6A / ACTL6B
Additional Names:
BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 complex subunit K, actin-related protein 4, hArpN beta
Protein Accession Number:
NP_004292.1,NP_817126.1,NP_057272.1
Protein Gi Number:
7705294
Ncbi Gene Id Number:
51412;86
Background Reference 1:
Harata M, Mochizuki R, Mizuno S. Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues. Biosci Biotechnol Biochem. 1999 May;63(5):917-23.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-314

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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