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A) HEK293 overexpressing BAF57 (RC209444) and probed with 45-315 (mock transfection in first lane), tested by Origene. B) see Western Blot.
In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5ug/ml.
A) HEK293 overexpressing BAF57 (RC209444) and probed with 45-315 (mock transfection in first lane), tested by Origene. B) see Western Blot.

BAF57 Antibody

CATALOG NUMBER: 45-315

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-PPTDPIPEDEKKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 ug/ml.Full caption:A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene.B) EB06054 staining (0.2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SMARCE1
Additional Names:
SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Protein Accession Number:
NP_003070.3
Protein Gi Number:
21264355
Ncbi Gene Id Number:
6605
Background Reference 1:
Wang W, Chi T, Xue Y, Zhou S, Kuo A, Crabtree GR. Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes. Proc Natl Acad Sci USA. 1998 Jan 20;95(2):492-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-315

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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