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45-318 (2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-318 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-318 (2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BAG5 Antibody

CATALOG NUMBER: 45-318

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DMGNQHPSISRLQ-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55+48kDa bands observed in nuclear lysates of cell line HeLa (calculated MW of 56.0kDa according to NP_001015049.1 and of 51.2kDa according to NP_004864.1; NP_001015048.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Specificity:
Variants (NP_004864.1; NP_001015048.1) encode the same isoform.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BAG5
Additional Names:
BAG5, BCL2-associated athanogene 5, BAG-5, KIAA0873, BAG-family molecular chaperone regulator-5
Protein Accession Number:
NP_001015049.1,NP_004864.1,NP_001015048.1
Protein Gi Number:
6631077
Ncbi Gene Id Number:
9529
Background Reference 1:
Takayama S, Xie Z, Reed JC. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J Biol Chem. 1999 Jan 8;274(2):781-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-318

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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