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45-321 staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-321 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-321 staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

BAP Antibody

CATALOG NUMBER: 45-321

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ELLGSVNSLLKELR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended concentration: 1-2ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Testis. Recommended concentration: 5ug/ml.
Specificity:
Variants (NP_071909.1; NP_001032722.1) encode the same isoform.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SIL1
Additional Names:
SIL1, BAP, endoplasmic reticulum chaperone SIL1, homolog of yeast, BiP-associated protein, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), MSS, ULG5, SIL1 protein
Protein Accession Number:
NP_071909.1,NP_001032722.1
Protein Gi Number:
11968009
Ncbi Gene Id Number:
64374
Background Reference 1:
Chung KT, Shen Y, Hendershot LM. BAP, a Mammalian BiP-associated Protein, Is a Nucleotide Exchange Factor That Regulates the ATPase Activity of BiP. J Biol Chem. 2002 Dec 6;277(49):47557-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-321

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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