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45-364 (1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Human CTNNA1 and probed with 45-364 (mock transfection in first lane).
45-364 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-364 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-364 (1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Catenin alpha 1 Antibody

CATALOG NUMBER: 45-364

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KREKQDETQTKIK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Prostate and Uterus. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CTNNA1
Additional Names:
catenin (cadherin-associated protein), alpha 1, 102kDa , HGNC:2509, CTNNA1, CAP102, FLJ36832 , alpha 1 (102kD), alpha-E-catenin, alpha-catenin, alphaE-catenin, cadherin-associated protein,102kDa, catenin (cadherin-associated protein), alpha 1 (102kD), catenin, alpha 1
Protein Accession Number:
NP_001894.2
Protein Gi Number:
55770844
Ncbi Gene Id Number:
1495
Background Reference 1:
Lynch HT, Grady W, Suriano G, Huntsman D. Gastric cancer: New genetic developments. J Surg Oncol. 2005 May 13;90(3):114-133

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-364

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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