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45-368 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-368 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-368 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-368 (0.1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-368 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CBR1 Antibody

CATALOG NUMBER: 45-368

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30-35kDa band observed in lysates of Glioblastoma cell line U251 and in Human Kidney lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration: 1-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CBR1
Additional Names:
CBR1, carbonyl reductase 1, CBR, carbonyl reductase (NADPH), carbonyl reductase (NADPH) 1, hCBR1, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1
Protein Accession Number:
NP_001748.1
Protein Gi Number:
4502599
Ncbi Gene Id Number:
873
Background Reference 1:
Balcz B, Kirchner L, Cairns N, Fountoulakis M, Lubec G Increased brain protein levels of carbonyl reductase and alcohol dehydrogenasein Down syndrome and Alzheimer's disease. J Neural Transm Suppl. 2001;(61):193-201

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-368

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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