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45-389 (1ug/ml) staining of Human Cerebellum (A), Mouse (B), Rat (C) and Pig (D) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-389 (1ug/ml) staining of Human Cerebellum (A), Mouse (B), Rat (C) and Pig (D) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CENTA1 Antibody

CATALOG NUMBER: 45-389

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QEYAVEAHFKHKP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), Mouse, Rat and Pig Brain lysates (calculated MW of 43.4kDa according to Human NP_006860.1, Mouse NP_766311.2, Rat NP_598251.1 and Pig NP_999391.1). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADAP1
Additional Names:
ADAP1, CENTA1, centaurin, alpha 1, GCS1L, p42IP4, centaurin-alpha, ArfGAP with dual PH domains 1
Protein Accession Number:
NP_006860.1
Protein Gi Number:
6806913
Ncbi Gene Id Number:
11033
Background Reference 1:
Venkateswarlu K, Oatey PB, Tavare JM, Jackson TR, Cullen PJ. Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3, 4, 5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1. Biochem J. 1999 Jun 1;340 ( Pt 2):359-63.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-389

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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