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45-435 (5ug/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-435 Immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (bl
45-435 Flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goa
45-435 (5ug/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Cryopyrin Antibody

CATALOG NUMBER: 45-435

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-EKPELTVVFEPSW
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:This antibody is not recommended for use in Western blotting.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of human peripheral blood monocyte cells. Recommended concentration: 10ug/ml.

Specificity:
Variants (NP_001073289.1; NP_004886.3) encode the same isoform.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NLRP3
Additional Names:
NLRP3, CIAS1, FCU, MWS, FCAS, NALP3, C1orf7, PYPAF1, AII/AVP, AGTAVPRL, CRYOPYRIN, cold autoinflammatory syndrome 1, cryopyrin, Muckle-Wells syndrome, chromosome 1 open reading frame 7, PYRIN-containing APAF1-like protein 1, neuronal apoptosis inhibitor protein 3, angiotensin/vasopressin receptor AII/AVP-like, NLR family, pyrin domain containing 3, AII, AVP, CLR1.1, FLJ95925, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Protein Accession Number:
NP_001073289.1,NP_004886.3,NP_899632.1,NP_001120933.1,NP_001120934.1
Protein Gi Number:
34878693
Ncbi Gene Id Number:
114548
Background Reference 1:
Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD. Mutation of a new gene encoding a putative pyrin-like protein causes familial cold autoinflammatory syndrome and Muckle-Wells syndrome. Nat Genet. 2001 Nov;29(3):301-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-435

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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