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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human EPM2AI with DYKDDDDK tag probed with 45-547 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 45-547 (1mg/ml) in Lane B. Primar
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human EPM2AI with DYKDDDDK tag probed with 45-547 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 45-547 (1mg/ml) in Lane B. Primar

EPM2A Antibody

CATALOG NUMBER: 45-547

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: WMTPKRSKMEVDC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human EPM2AIP1 (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti- DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EPM2AIP1
Additional Names:
EPM2AIP1, FLJ11207, KIAA0766, EPM2A (laforin) interacting protein 1, laforin interacting protein 1, EPM2A interacting protein 1
Protein Accession Number:
NP_055620.1
Protein Gi Number:
7662294
Ncbi Gene Id Number:
9852
Background Reference 1:
Chan EM, Ackerley CA, Lohi H, Ianzano L, Cortez MA, Shannon P, Scherer SW, Minassian BA. Laforin preferentially binds the neurotoxic starch-like polyglucosans, which form in its absence in progressive myoclonus epilepsy. Hum Mol Genet. 2004 Jun 1;13(11):1117-29. Epub 2004 Apr 21.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-547

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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