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42-817 (0.01ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-817 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-817 (0.01ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UCHL1 Antibody

CATALOG NUMBER: 42-817

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: EVRFSAVALCK
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Brain (Hippocampus) and Mouse and Rat Brain lysates (calculated MW of 24.8kDa according to Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB10219.
Positive Control 1:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Positive Control 2:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
UCHL1
Additional Names:
UCHL1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), PARK5, PGP9.5, PGP95, Uch-L1, neuron cytoplasmic protein 9.5, ubiquitin C-terminal hydrolase, ubiquitin carboxyl-terminal esterase L1
Protein Accession Number:
NP_004172.2
Protein Gi Number:
21361091
Ncbi Gene Id Number:
7345

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-817

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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