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46-331 (2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-331 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-331 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-331 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-331 (2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Sak Antibody

CATALOG NUMBER: 46-331

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: ATCIGEKIEDFKVGN
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Colon lysates (calculated MW of 109kDa according to NP_055079.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PLK4
Additional Names:
PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase, STK18, serine/threonine protein kinase SAK, MCCRP2, STK 18
Protein Accession Number:
NP_055079.3,NP_001177728.1
Protein Gi Number:
21361433
Ncbi Gene Id Number:
10733
Background Reference 1:
Hudson JW, Chen L, Fode C, Binkert C, Dennis JW. Sak kinase gene structure and transcriptional regulation. Gene. 2000 Jan 4;241(1):65-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-331

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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