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46-336 (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing SAR1B and probed with 46-336 (mock transfection in first lane).
46-336 (0.03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SAR1B Antibody

CATALOG NUMBER: 46-336

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-DHERLLESKEELD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 25kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.
Specificity:
Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SAR1B
Additional Names:
SAR1B, SARA2, SAR1 gene homolog B (S. cerevisiae), CMRD, GTBPB, 2310075M17Rik, GTP-binding protein Sara, SAR1a gene homolog 2
Protein Accession Number:
NP_001028675.1,NP_057187.1
Protein Gi Number:
75709204
Ncbi Gene Id Number:
51128
Background Reference 1:
Evans K, Burdge GC, Wootton SA, Collins JM, Clark ML, Tan GD, Karpe F, Frayn KN. Tissue-specific stable isotope measurements of postprandial lipid metabolism in familial combined hyperlipidaemia. Atherosclerosis. 2007 Apr 25;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-336

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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