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46-341 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing SKAP2 and probed with 46-341 (mock transfection in first lane).
46-341 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SCAP2 Antibody

CATALOG NUMBER: 46-341

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GLVPKAYIMEMYDI
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2ug/ml.An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SKAP2
Additional Names:
SKAP2, SCAP2, PRAP, src family associated phosphoprotein 2, MGC10411, MGC33304, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, src family associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adaptor protein
Protein Accession Number:
NP_003921.2
Protein Gi Number:
16753212
Ncbi Gene Id Number:
8935
Background Reference 1:
Takahashi T, Yamashita H, Nagano Y, Nakamura T, Ohmori H, Avraham H, AvrahamS, Yasuda M, Matsumoto M. Identification and characterization of a novel Pyk2/related adhesion focaltyrosine kinase-associated protein that inhibits alpha-synucleinphosphorylation. J Biol Chem. 2003 Oct 24;278(43):42225-33. Epub 2003 Jul 31

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-341

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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