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46-376 (2ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-376 (2ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SLC24A5 Antibody

CATALOG NUMBER: 46-376

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: TTPDCRKKFWKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 40kDa band observed in Mouse Eye and Rat Skin lysates (calculated MW of 54.9 according to Human NP_995322.1, of 55.0kDa according to Mouse NP_778199.2 and of 38.1kDa according to Rat NP_001101239.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ginger et al, J Biol Chem. 2008 Feb 29;283(9):5486-95; PMID: 18166528). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1408 - Mouse Eye Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC24A5
Additional Names:
SLC24A5 , solute carrier family 24,member 5 , JSX, NCKX5 , ion transporter JSX, solute carrier family 24 (sodium/potassium/calcium exchanger),member 5
Protein Accession Number:
NP_995322.1
Protein Gi Number:
45504369
Ncbi Gene Id Number:
283652
Background Reference 1:
Lamason RL, Mohideen MA, Mest JR, Wong AC, Norton HL, Aros MC, Jurynec MJ, Mao X, Humphreville VR, Humbert JE, Sinha S, Moore JL, Jagadeeswaran P, Zhao W, Ning G, Makalowska I, McKeigue PM, O'donnell D, Kittles R, Parra EJ, Mangini NJ, Grunwald DJ, Shriver MD, Canfield VA, Cheng KC. SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans. Science. 2005 Dec 16;310(5755):1782-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-376

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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