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46-387 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-387 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SMN1 Antibody

CATALOG NUMBER: 46-387

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DESENSRSPGNKSDN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 28.5kDa according to NP_075012.1 and NP_075014.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SMN1 / SMN2
Additional Names:
SMN1, survival of motor neuron 1, telomeric, BCD541, SMA1, SMA2, SMA3, SMA4, SMN, SMNT, T-BCD541, gemin 1, SMN2, survival of motor neuron 2, centromeric, BCD541, C-BCD541, SMNC,
Protein Accession Number:
NP_075012.1,NP_000335.1,NP_075013.1,NP_075014.1,NP_075015.1,NP_059107.1
Protein Gi Number:
13259512
Ncbi Gene Id Number:
6606;6607
Background Reference 1:
Setola V, Terao M, Locatelli D, Bassanini S, Garattini E, Battaglia G. Axonal-SMN (a-SMN), a protein isoform of the survival motor neuron gene, is specifically involved in axonogenesis. Proc Natl Acad Sci USA. 2007 Feb 6;104(6):1959-64. Epub 2007 Jan 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-387

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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