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46-440 (1ug/ml) staining of Human Testis Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-440 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-440 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-440 (1ug/ml) staining of Human Testis Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Stathmin 1 Antibody

CATALOG NUMBER: 46-440

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-KNKESKDPADETEAD
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 17kDa band observed in Human Testis and Mouse Brain lysates (calculated MW of 17.3kDa according to NP_005554.1, NP_981944.1 and NP_981946.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Thymus and Tonsil. Recommended concentration: 3.75ug/ml.ELISA: This antibody has been successfully used in ELISA as described in the following paper: Song et al, Aging Cell. 2010 Aug;9(4):607-15; PMID: 20560902.
Specificity:
This antibody is expected to cross-react with isoform 1 a (NP_005554.1; NP_981944.1; NP_981946.1) only. The three reported isoforms (NP_005554.1, NP_981944.1 and NP_981946.1) represent identical protein.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STMN1
Additional Names:
STMN1, stathmin 1/oncoprotein 18 , LAP18, Lag, OP18, PP17, PP19, PR22, SMN , Pr22 protein, leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18, phosphoprotein 19, prosolin, stathmin 1, stathmin 1 variant
Protein Accession Number:
NP_005554.1,NP_981944.1,NP_981946.1
Protein Gi Number:
5031851
Ncbi Gene Id Number:
3925
Background Reference 1:
Miyashita H, Kanemura M, Yamazaki T, Abe M, Sato Y. Vascular endothelial zinc finger 1 is involved in the regulation of angiogenesis: possible contribution of stathmin/OP18 as a downstream target gene. Arterioscler Thromb Vasc Biol. 2004 May;24(5):878-84. Epub 2004 Mar 18.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-440

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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