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46-443 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-443 (2.5ul) staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.
46-443 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-443 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Stearoyl-CoA desaturase Antibody

CATALOG NUMBER: 46-443

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RIKRTGDGNYKSG
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SCD
Additional Names:
SCD, SCD1, HGNC:10571, FADS5, PRO0998 , acyl-CoA desaturase, delta-9-desaturase, fatty acid desaturase, predicted protein of HQ0998, stearoyl-CoA desaturase
Protein Accession Number:
NP_005054.3
Protein Gi Number:
53759151
Ncbi Gene Id Number:
6319
Background Reference 1:
Zhang S, Yang Y, Shi Y. Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J. 2004 Dec 20;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-443

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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