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46-495 (2ug/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-495 (1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-495 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic and nuclear staining. The nu
46-495 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
46-495 (2ug/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TIM-3 Antibody

CATALOG NUMBER: 46-495

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KWYSHSKEKIQN
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell lines Jurkat and MOLT-4 and in Human Tonsil lysates (calculated MW of 33.4kDa according to NP_116171.3). Recommended concentration:1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 (squamous carcinoma) cell lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HAVCR2
Additional Names:
HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3
Protein Accession Number:
NP_116171.3
Protein Gi Number:
49574534
Ncbi Gene Id Number:
84868
Background Reference 1:
Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases. Trends Mol Med. 2005 Aug;11(8):362-9. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-495

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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