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46-506 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-506 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-506 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TPD52 Antibody

CATALOG NUMBER: 46-506

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TEPLPEKTQES
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26-28kDa band observed in Human Lymph Node and Human Kidney lysates (calculated MW of 24.3kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tiacci et al , Blood. 2005 Apr 1;105(7):2812-20.; PMID: 15576473). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise all three reported isoforms (NP_001020423.1, NP_001020424.1and NP_005070.1).
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TPD52
Additional Names:
TPD52, tumor protein D52, D52, N8L, PC-1, PrLZ, hD52, PC1/MRPS28 fusion protein, prostate and colon associated protein
Protein Accession Number:
NP_001020423.1,NP_001020424.1,NP_005070.1
Protein Gi Number:
70608172
Ncbi Gene Id Number:
7163
Background Reference 1:
Byrne JA, Balleine RL, Schoenberg Fejzo M, Mercieca J, Chiew YE, Livnat Y, St Heaps L, Peters GB, Byth K, Karlan BY, Slamon DJ, Harnett P, Defazio A. Tumor protein D52 (TPD52) is overexpressed and a gene amplification target in ovarian cancer. Int J Cancer. 2005 Dec 20;117(6):1049-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-506

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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